Jalview 2.10.0
Version Date:
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New Features
General
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Updated Spanish translations.
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Jmol now primary parser for importing structure data to Jalview. Enables mmCIF and better PDB parsing.
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Alignment ruler shows positions relative to reference sequence
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Position/residue shown in status bar when mousing over sequence associated annotation
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Default RNA SS symbol to ‘matching bracket’ for manual entry
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RNA Structure consensus indicates wc-only ‘()’, canonical ‘[]’ and invalid ‘{}’ base pair populations for each column
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Feature settings popup menu options for showing or hiding columns containing a feature
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Edit selected group by double clicking on group and sequence associated annotation labels
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Sequence name added to annotation label in select/hide columns by annotation and colour by annotation dialogs
Application
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Automatically hide introns when opening a gene/transcript view
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Uniprot Sequence fetcher Free Text Search dialog
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UniProt - PDB protein structure mappings with the EMBL-EBI PDBe SIFTS database
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Updated download sites used for Rfam and Pfam sources to xfam.org
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Disabled Rfam(Full) in the sequence fetcher
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Show residue labels in Chimera when mousing over sequences in Jalview
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Support for reverse-complement coding regions in ENA and EMBL
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Upgrade to EMBL XML 1.2 for record retrieval via ENA rest API
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Support for ENA CDS records with reverse complement operator
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Update to groovy-2.4.6-indy - for faster groovy script execution
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New ’execute Groovy script’ option in an alignment window’s Calculate menu
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Allow groovy scripts that call Jalview.getAlignFrames() to run in headless mode
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Support for creating new alignment calculation workers from groovy scripts
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Store/restore reference sequence in Jalview projects
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Chain codes for a sequence’s PDB associations are now saved/restored from project
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Database selection dialog always shown before sequence fetcher is opened
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Double click on an entry in Jalview’s database chooser opens a sequence fetcher
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Free-text search client for UniProt using the UniProt REST API
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-nonews command line parameter to prevent the news reader opening
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Displayed columns for PDBe and Uniprot querying stored in preferences
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Pagination for displaying PDBe and Uniprot search results
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Tooltips shown on database chooser
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Reverse complement function in calculate menu for nucleotide sequences
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Alignment sort by feature scores and feature counts preserves alignment ordering (and debugged for complex feature sets).
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Chimera 1.11.1 minimum requirement for viewing structures with Jalview 2.10
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Retrieve genome, transcript CCDS and gene ids via the Ensembl and Ensembl Genomes REST API
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Protein sequence variant annotation computed for ‘sequence_variant’ annotation on CDS regions (Ensembl)
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ENA CDS ‘show cross references’ for Uniprot sequences
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Improved warning messages when DB Ref Fetcher fails to match, or otherwise updates sequence data from external database records.
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Revised Jalview Project format for efficient recovery of sequence coding and alignment annotation relationships.
Issues Resolved
General
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reinstate CTRL-click for opening pop-up menu on OSX
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Export features in Jalview format (again) includes graduated colourschemes
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More responsive when working with big alignments and lots of hidden columns
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Hidden column markers not always rendered at right of alignment window
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Tidied up links in help file table of contents
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Feature based tree calculation not shown for DNA alignments
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Hidden columns ignored during feature based tree calculation
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Alignment view stops updating when show unconserved enabled for group on alignment
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Cannot insert gaps into sequence when set as reference
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Alignment column in status incorrectly shown as “Sequence position” when mousing over annotation
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Incorrect column numbers in ruler when hidden columns present
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Colour by RNA Helices not enabled when user created annotation added to alignment
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RNA Structure consensus only computed for ‘()’ base pair annotation
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Enabling ‘Ignore Gaps’ results in zero scores for all base pairs in RNA Structure Consensus
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Extend selection with columns containing feature not working
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Pfam format writer puts extra space at beginning of sequence
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Incomplete sequence extracted from pdb entry 3a6s
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Cannot create groups on an alignment from from a tree when t-coffee scores are shown
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Cannot import and view PDB structures with chains containing negative resnums (4q4h)
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ArithmeticExceptions raised when parsing some structures
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‘Empty’ alignment blocks added to Clustal, PIR and PileUp output
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Reordering sequence features that are not visible causes alignment window to repaint
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Threshold sliders don’t work in graduated colour and colour by annotation row for e-value scores associated with features and annotation rows
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amino acid physicochemical conservation calculation should be case independent
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Remove annotation also updates hidden columns
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FER1_ARATH and FER2_ARATH mislabelled in example file (uniref50.fa, feredoxin.fa, unaligned.fa, exampleFile_2_7.jar, exampleFile.jar, exampleFile_2_3.jar)
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Null pointer exceptions and redraw problems when reference sequence defined and ‘show non-conserved’ enabled
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Quality and Conservation are now shown on load even when Consensus calculation is disabled
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Remove right on penultimate column of alignment does nothing
Application
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URLs and links can’t be imported by drag’n’drop on OSX when launched via webstart (note - not yet fixed for El Capitan)
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Corrupt preferences for SVG, EPS & HTML output when running on non-gb/us i18n platforms
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Error thrown when exporting a view with hidden sequences as flat-file alignment
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InstallAnywhere distribution fails when launching Chimera
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Jalview very slow to launch via webstart (also hotfix for 2.9.0b2)
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Cannot save project when view has a reference sequence defined
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Columns are suddenly selected in other alignments and views when revealing hidden columns
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Hide columns not mirrored in complement view in a cDNA/Protein splitframe
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Cannot save/restore representative sequence from project when only one sequence is represented
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Disabled ‘Best Uniprot Coverage’ option in Structure Chooser
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Modifying ‘Ignore Gaps’ on consensus or structure consensus didn’t refresh annotation panel
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View mapping in structure view shows mappings between sequence and all chains in a PDB file
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PDB and Uniprot FTS dialogs format columns correctly, don’t display array data, sort columns according to type
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Export complete shown after destination file chooser is cancelled during an image export
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Error when querying PDB Service with sequence name containing special characters
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Manual PDB structure querying should be case insensitive
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Large tooltips with broken HTML formatting don’t wrap
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Figures exported from wrapped view are truncated so L looks like I in consensus annotation
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Export features should only export the currently displayed features for the current selection or view
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Enable ‘Get Cross-References’ in menu after fetching cross-references, and restoring from project
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Mouseover of a copy of a sequence is not followed in the structure viewer
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Titles for individual alignments in splitframe not restored from project
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missing autocalculated annotation at trailing end of protein alignment in transcript/product splitview when pad-gaps not enabled by default
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amino acid physicochemical conservation is case dependent
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RSS reader doesn’t stay hidden after last article has been read (reopened issue due to internationalisation problems)
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Only offer PDB structures in structure viewer based on sequence name, PDB and UniProt cross-references
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No progress bar shown during export of alignment as HTML
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Structures not always superimposed after multiple structures are shown for one or more sequences.
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Reference sequence characters should not be replaced with ‘.’ when ‘Show unconserved’ format option is enabled.
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Cannot specify chain code when entering specific PDB id for sequence
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File->Export->.. as doesn’t work when ‘Export hidden sequences’ is enabled, but ’export hidden columns’ is disabled.
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Best Quality option in structure chooser selects lowest rather than highest resolution structures for each sequence
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Incorrect start and end reported for PDB to sequence mapping in ‘View Mappings’ report
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Unable to read old Jalview projects that contain non-XML data added after Jalvew wrote project.
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Newly created annotation row reorders after clicking on it to create new annotation for a column.
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Null Pointer Exception raised when pressing Add on an orphaned cut’n’paste window.